← Resources

Care Guide

Tissue Culture Clone Care Sheet

Tissue culture clones are more vigorous than traditional clones but need special care. This guide covers receiving, acclimating, and transplanting them — with the details that keep these robust-yet-delicate plants thriving.

Tissue culture clones are more vigorous than traditional clones, but they require special care because of their size and shipping container. This care sheet walks you through receiving, acclimating, transplanting, and preparing them for the vegetative phase.

Receiving Tissue Culture Clones

  1. 1
    Visual inspection:
    • Check that the container is sealed and undamaged.
    • Confirm the clones inside are intact and healthy.
  2. 2
    Sanitize the container:
    • Spray with 70% alcohol before starting the acclimation process.
  3. 3
    Initial setup (before acclimation):
    • Place clones under clone lights (6500K–9500K, 90–120 PPFD).
    • Set humidity to 70%–75%.
    • Temperature: 78°F–80°F.
    • Let the clones rest for at least 4–8 hours (preferably 24 hours) to recover from shipping stress.

Acclimation Process

  1. 1
    Environmental conditions:
    • Continue using the same clone lights, humidity (70%–75%), and temperature (78°F–80°F).
  2. 2
    Step-by-step air exposure:
    • Step 1: Open the container by peeling back 1/3 of the seal, then wait 12–24 hours.
    • Step 2: Peel back the next 1/3 and wait another 12–24 hours.
    • Step 3: If the clones appear healthy (not stressed), remove the entire seal.
  3. 3
    Feeding clones:
    • Start with a vegetative nutrient solution (EC 1.0–2.5 — Endless runs 2.7–3.0 EC).
    • pH: 5.8–5.9.
    • Let them remain in this environment for 3–5 days.

Optional Steps for Healthier Clones

  1. 1
    Inoculation:
    • Use Trichoderma to protect against pathogens like Pythium and Fusarium.
  2. 2
    Foliar spraying:
    • If the clones appear stressed, you may use a light foliar spray during acclimation.

Transplanting Tissue Culture Clones

Tissue culture clones are smaller than traditional clones and need careful handling. Allow extra time for acclimation to their new environment, as they're still in early developmental stages.

  1. 1
    General conditions:
    • Temperature: 78°F–82°F.
    • Humidity: 60%–75%.
    • Nutrients (Rockwool or coco):
      • EC: 1.5–3.0.
      • pH: 5.8–5.9.

Transplanting into Rockwool

  1. 1
    Prepare the Rockwool:
    • Soak in pH-adjusted water (5.7–6.0) for 30–60 minutes.
  2. 2
    Check clone health:
    • Ensure roots are healthy and the clone is fully acclimated to a non-sterile environment.
  3. 3
    Transplant:
    • Create a hole in the Rockwool, place the clone, and gently press to secure it.
  4. 4
    Water and monitor:
    • Water lightly with a pH-balanced solution and monitor moisture levels.

Transplanting into Coco Coir

  1. 1
    Prepare the coco:
    • Ensure it is pre-buffered and moist, but not waterlogged.
  2. 2
    Check clone health:
    • Confirm the clone has healthy roots and is acclimated to its environment.
  3. 3
    Transplant:
    • Create a hole, use rooting gel if desired, and gently place the clone.
  4. 4
    Water and monitor:
    • Use a pH-balanced nutrient solution (pH 5.8–6.0) and make sure calcium and magnesium are adequate.

In Closing

Monitor the clones closely through acclimation and transplanting to set them up for success. The gradual process helps tissue culture clones thrive in their new environment. Questions along the way? Our team is one message away.